Journal: bioRxiv

Article Title: Regulation and Function of the HPV16 CircE7 RNA

doi: 10.64898/2026.03.09.710444

Figure Lengend Snippet: (A) RT-qPCR of circE7 and E6*I normalized to linear E6 RNA from primary keratinocytes transfected with HPV16 WT or HPV16 mut2 genomes (circE7 splicing acceptor m 6 A motif mutation, no change to AA sequence). *p<0.05, ****p<0.0001. (B) Western blot of p53, GAPDH and HSP90 in whole cell extracts from Keratinocytes transfected with HPV16 WT or mut2 genomes. (C) Viral DNA detected by qPCR after HIRT DNA extraction out of primary Keratinocytes transfected with either wild type or mut2 HPV16. ****p<0.0001. (D) Crystal Violet staining of keratinocytes grown in 10% BCS DMEM for 2-6 weeks. Cells were transfected with either wild type HPV16 or HPV16 Mut2. Representative images (left) and quantification at week 6 (right). ***p<0.001

Article Snippet: Briefly, early passage primary keratinocytes were co-transfected with an equal DNA ratio of either pHPV16ANsL WT or Mut2 and pCAGGS-nlsCre plasmids (gift from CM Chiang lab) using TransIT Keratinocyte Transfection Reagent (MirusBio, MIR 2800) according to the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Transfection, Mutagenesis, Sequencing, Western Blot, DNA Extraction, Staining